Rodriguez-Concepcion M, Avalos J, Bonet ML, Boronat A, Gomez-Gomez L, Hornero-Mendez D, Limon MC, Meléndez-Martínez AJ, Olmedilla-Alonso B, Palou A, Ribot J, Rodrigo MJ, Zacarias L, Zhu C (2018) A global perspective on carotenoids: metabolism, biotechnology, and benefits for nutrition and health.Vishnevetsky M, Ovadis M, Vainstein A (1999) Carotenoid sequestration in plants: the role of carotenoid-associated proteins.Li L, Yuan H, Zeng Y, Xu Q (2016) Plastids and carotenoid accumulation.Li L, Yuan H (2013) Chromoplast biogenesis and carotenoid accumulation.Egea I, Barsan C, Bian W, Purgatto E, Latché A, Chervin C, Bouzayen M, Pech JC (2010) Chromoplast differentiation: current status and perspectives.The fractions obtained are suitable for metabolomics and proteomics analysis. Here we describe a combined protocol for the isolation, lysis and fractionation of tomato fruit chromoplast.
The combination of classical fractionation methods with the use of biochemical and -omics techniques represents an attractive approach to unravel novel aspects related with the biochemical and cellular mechanisms underlying the biogenesis of the structures involved in the biosynthesis and storage of carotenoids during chromoplast differentiation. At present, very little is known about chromoplast biogenesis and the role of the different suborganellar structures in the synthesis and storage of carotenoids. These subplastidial structures show remarkable morphological differences and seem to be adapted to the accumulation of particular carotenoids in some plant species and organs. Chromoplast differentiation involves an active synthesis of carotenoids associated with the remodeling of the preexisting plastid membrane systems to form specialized structures involved in the sequestration and storage of the synthesized carotenoids.
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